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Updated: Oct 31, 2025

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LncRNA X inactive-specific transcript promotes osteoclast differentiation through Tgif2 by acting as a ceRNA of

Yuefeng Shao1,2, Xinya Hu3, Xuejian Wu1

  • 1Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.

Regenerative Medicine
|June 30, 2021
PubMed

Insights

Long noncoding RNA XIST promotes osteoclast differentiation in osteoporosis by acting as a ceRNA for miR-590-3p, upregulating Tgif2. This finding offers potential therapeutic targets for osteoporosis treatment.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Bone Biology

Background:

  • Osteoporosis is characterized by decreased bone mass and increased fracture risk.
  • Osteoclast differentiation plays a critical role in bone resorption and osteoporosis pathogenesis.
  • Long noncoding RNAs (lncRNAs) are emerging as key regulators in various biological processes, including bone metabolism.

Purpose of the Study:

  • To investigate the role of long noncoding RNA X-inactive specific transcript (Xist) in regulating osteoclast differentiation in osteoporosis.
  • To elucidate the underlying molecular mechanism involving Xist, miR-590-3p, and Tgif2.

Main Methods:

  • Established a mouse model of osteoporosis via ovariectomy.
  • Induced osteoclast differentiation from RAW264.7 cells in vitro.
  • Assessed gene expression and regulatory relationships using molecular biology techniques.

Main Results:

  • Xist and Tgif2 were upregulated, while miR-590-3p was downregulated in osteoporosis models.
  • Xist knockdown or miR-590-3p overexpression inhibited Tgif2 expression and osteoclast differentiation.
  • Xist functions as a competing endogenous RNA (ceRNA) for miR-590-3p, thereby promoting Tgif2 expression and osteoclast differentiation.

Conclusions:

  • Xist promotes osteoclast differentiation in osteoporosis by sponging miR-590-3p and upregulating Tgif2.
  • The Xist/miR-590-3p/Tgif2 axis represents a potential therapeutic target for osteoporosis.
Keywords:
Tgif2lncRNA XistmiR-590-3posteoclast differentiationosteoporosis

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