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Updated: Oct 31, 2025

Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy
Published on: January 7, 2019
Spatio-temporal ultrasound beam modulation to sequentially achieve multiple foci with a single planar monofocal lens
Sergio Pérez-López1, José Miguel Fuster1, Pilar Candelas2
1Centro de Tecnologías Físicas, Universitat Politècnica de València, 46022, Valencia, Spain.
Abstract:
Ultrasound focusing is a hot topic due to its multiple applications in many fields, including biomedical imaging, thermal ablation of cancerous tissues, and non destructive testing in industrial environments. In such applications, the ability to control the focal distance of the ultrasound device in real-time is a key advantage over conventional devices with fixed focal parameters. Here, we present a method to achieve multiple time-modulated ultrasound foci using a single planar monofocal Fresnel Zone Plate. The method takes advantage of the focal distance linear dependence on the operating frequency of this kind of lenses to design a sequence of contiguous modulated rectangular pulses that achieve different focal distances and intensities as a function of time. Both numerical simulations and experimental results are presented, demonstrating the feasibility and potential of this technique.

