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Decreased TMEM40 expression is associated with malignant behavior of cutaneous squamous cell carcinoma and inhibits

Lei Yu1, Jie Liu2, Tang-De Zhang1

  • 1Department of Dermatology, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong 510282, P.R. China.

Oncology Letters
|June 30, 2021
PubMed

Insights

Transmembrane protein 40 (TMEM40) is elevated in cutaneous squamous cell carcinoma (CSCC). Reducing TMEM40 inhibits CSCC cell growth, migration, and invasion, suggesting it

Area of Science:

  • Oncology
  • Molecular Biology
  • Dermatology

Background:

  • Cutaneous squamous cell carcinoma (CSCC) is a prevalent human skin cancer.
  • Understanding cancer-associated transmembrane proteins is crucial for CSCC molecular biology.
  • Transmembrane protein 40 (TMEM40) role in CSCC requires investigation.

Purpose of the Study:

  • To evaluate TMEM40 expression patterns in CSCC.
  • To determine the clinical significance of TMEM40 in CSCC.
  • To elucidate the underlying mechanisms of TMEM40 in CSCC progression.

Main Methods:

  • Quantitative PCR, Western blot, and immunohistochemistry for TMEM40 expression analysis.
  • Cell Counting Kit-8 assays for proliferation assessment.
  • Wound healing, flow cytometry, and Transwell assays for migration, cell cycle, apoptosis, and invasion studies.

Main Results:

  • TMEM40 expression is significantly increased in CSCC tissues compared to normal skin.
  • Elevated TMEM40 expression correlates with larger tumor size in CSCC patients.
  • TMEM40 silencing in CSCC cells induced G0/G1 cell cycle arrest, promoted apoptosis, and inhibited migration and invasion.

Conclusions:

  • TMEM40 plays a role in regulating CSCC cell growth, cell cycle, apoptosis, migration, and invasion.
  • Reduced TMEM40 expression inhibits CSCC development.
  • TMEM40 represents a potential therapeutic target for CSCC treatment.
Keywords:
CSCCTMEM40apoptosisbiomarkermigration

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