Related Experiment Video
Updated: Jun 19, 2026
A Double Humanized BLT-mice Model Featuring a Stable Human-Like Gut Microbiome and Human Immune System
Published on: August 30, 2019
Gut microbiota transplantation for colonization of germ-free mice
Jocelyn M Choo1,2, Geraint B Rogers1,2
1Microbiome and Host Health, South Australia Health and Medical Research Institute, Adelaide 5000, Australia.
Abstract:
The use of germ-free mice is integral to the understanding of host-gut microbiome relationships. Such models rely on faithful replication of the donor microbiome to establish causal effects of the gut microbiota on host pathophysiology. This protocol describes the preparation and transfer of donor microbiota, focusing on strict anaerobic processing methods and multiple instillations by gavage for optimal gut microbiota recovery. For complete details on the generation and use of this protocol, please refer to Choo and Rogers (2021).
