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Expression and Clinical Significance of BCL2 Interacting Protein 3 Like in Multiple Myeloma

Ruolin Li1, Gang Chen2, Yiwu Dang2

  • 1Department of Scientific Research, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.

Technology in Cancer Research & Treatment
|June 30, 2021
PubMed

Insights

BCL-2/adenovirus E1B 19 kDa-interacting protein 3-like (BNIP3L) is significantly downregulated in multiple myeloma (MM) patients. Lower BNIP3L levels indicate a protective factor for MM patient survival, potentially linked to the adipocytokine signaling pathway.

Area of Science:

  • Hematology
  • Oncology
  • Molecular Biology

Background:

  • Multiple myeloma (MM) is a significant hematologic malignancy.
  • Understanding the molecular mechanisms underlying MM pathogenesis is crucial for developing effective therapies.

Purpose of the Study:

  • To investigate the expression levels of BCL-2/adenovirus E1B 19 kDa-interacting protein 3-like (BNIP3L) in patients with multiple myeloma.
  • To explore the potential mechanisms and prognostic significance of BNIP3L in MM.

Main Methods:

  • Immunohistochemistry was used to analyze BNIP3L protein expression in bone marrow samples from MM patients and healthy donors.
  • Publicly available microarray and RNA sequencing data were analyzed to assess BNIP3L mRNA expression and its prognostic value.
  • Bioinformatic analyses, including gene co-expression, enrichment, and protein-protein interaction (PPI) analyses, were performed to identify associated signaling pathways.

Main Results:

  • BNIP3L expression was significantly lower in the bone marrow of MM patients compared to healthy donors at both protein and mRNA levels.
  • Meta-analysis of 694 MM cases revealed a significant downregulation of BNIP3L (SMD = -0.62) with high diagnostic accuracy (AUC = 0.81).
  • Lower BNIP3L levels were identified as an independent protective factor for MM patient survival (HR = 0.79).
  • Co-expression analysis identified 261 genes associated with BNIP3L, primarily involved in the adipocytokine signaling pathway.

Conclusions:

  • Downregulation of BNIP3L plays a potential role in the occurrence and development of multiple myeloma.
  • The oncogenic capacity associated with BNIP3L downregulation may be linked to the adipocytokine signaling pathway.
  • BNIP3L serves as a potential prognostic biomarker for multiple myeloma.
Keywords:
BNIP3Limmunohistochemistrymultiple myelomasilicosuppressor gene

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