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Monitoring of Nanodrug Accumulation in Murine Breast Cancer Metastases

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Long Non-Coding RNA NORAD Inhibits Breast Cancer Cell Proliferation and Metastasis by Regulating miR-155-5p/SOCS1

Weipeng Liu1, Xin Zhou1, Yuanqiang Li1

  • 1Department of General Surgery, The First College of Clinical Medical Science, China Three Gorges University, Yichang Central People's Hospital, Yichang, China.

Journal of Breast Cancer
|June 30, 2021
PubMed
Abstract

Insights

Non-coding RNA activated by DNA damage (NORAD) suppresses breast cancer progression by inhibiting microRNA-155-5p, leading to increased suppressor of cytokine signaling 1. This finding offers new insights into breast cancer (BC) treatment strategies.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) play crucial roles in cancer development.
  • Non-coding RNA activated by DNA damage (NORAD) is implicated in various cancers, but its function in breast cancer (BC) remains unclear.

Purpose of the Study:

  • To investigate the role of NORAD in breast cancer (BC).
  • To elucidate the molecular mechanism of NORAD in regulating BC cell malignancy.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) for gene expression analysis.
  • Western blotting for protein expression.
  • Cell proliferation, migration, and invasion assays (CCK-8, BrdU, Transwell).
  • Bioinformatics, RNA immunoprecipitation (RIP), and dual-luciferase reporter assays to confirm molecular interactions.

Main Results:

  • NORAD expression was significantly downregulated in BC tissues and cell lines, correlating with poor differentiation.
  • NORAD overexpression inhibited BC cell proliferation, migration, and invasion, while knockdown promoted these processes.
  • NORAD directly targeted microRNA (miR)-155-5p, and miR-155-5p targeted suppressor of cytokine signaling 1 (SOCS1).
  • NORAD suppressed miR-155-5p, leading to SOCS1 upregulation.

Conclusions:

  • NORAD acts as a tumor suppressor in breast cancer (BC).
  • The NORAD/miR-155-5p/SOCS1 axis regulates BC cell proliferation, migration, and invasion.
  • NORAD suppresses miR-155-5p to upregulate SOCS1, thereby inhibiting BC progression.
Keywords:
Breast neoplasmsCell proliferationMetastasis

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