Strategies of Prevascularization in Tissue Engineering and Regeneration of Craniofacial Tissues

Craniofacial tissue defects caused by trauma, developmental malformation, or surgery are critical issues of high incidence, which are harmful to physical and psychological health. Transplantation of engineered tissues or biomaterials is a potential method to repair defects and regenerate the craniofacial tissues. Revascularization is essential to ensure the survival and regeneration of the grafts. Since microvessels play a critical role in blood circulation and substance exchange, the pre-establishment of the microvascular network in transplants provides a technical basis for the successful regeneration of the tissue defect. In this study, we reviewed the recent development of strategies and applications of prevascularization in tissue engineering and regeneration of craniofacial tissues. We focused on the cellular foundation of the in vitro prevascularized microvascular network, the cell source for prevascularization, and the strategies of prevascularization. Several key strategies, including coculture, microspheres, three-dimensional printing and microfluidics, and microscale technology, were summarized and the feasibility of these technologies in the clinical repair of craniofacial defects was discussed. Impact statement Prevascularization is an effective strategy to promote the survival and regeneration of the graft after transplantation. This review focuses on recent development in the technique of prevascularization and discusses the advantages and limits of current technologies. Furthermore, we summarized the progress in the repair of craniofacial tissue defect with prevascularization strategies. The development raised promising prospects of prevascularization strategies in the regeneration of cranial facial tissue defects in the future.