Occurrence of 40 S.polysomal complexes in polysome profiles of reticulocyte lysates

In most reticulocyte lysates 40 S.polysomal complexes have such a short lifetime that they will not show up in the polysome profile. Here we describe a reticulocyte lysate where these 40 S.polysomal complexes apparently have a highly increased lifetime and therefore these complexes can be seen in the polysome profile as shoulders on the di-, tri- and tetrasome peak. The presence of these complexes in lysates probably signals an increased speed in the association of 40 S subunits with mRNA since similar alterations as described above show up in the polysome profile of normal lysates to which native ribosomal 40 S subunits were added.