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Published on: October 30, 2018
Neuroprotective effects on amyloid-beta induced cytotoxicity of Pandanus clementis Merr
Mario A Tan1, Byron Leander U Tan1, Maribel G Nonato1
1College of Science and Research Center for the Natural and Applied Sciences, University of Santo Tomas, 1015 Manila, Philippines.
Abstract:
The present study determined the neuroprotective potential of the alcoholic and aqueous extracts of Pandanus clementis Merr. (Pandanaceae) to protect the neuroblastoma SH-SY5Y cells against amyloid-beta 1-42 (Aβ) cytotoxicity. Inhibition of Aβ aggregation was determined by Thioflavin T (ThT) assay, and in vitro neuroprotective cell viability, intracellular reactive oxygen species (ROS), and mitochondrial membrane potential (MMP) were evaluated with human neuroblastoma SH-SY5Y cells insulted with Aβ. Chromatographic separation on the alcoholic extract yielded known phytosterols. Results showed that pretreatment of the SH-SY5Y cells with the P. clementis extracts increased cell viability and MMP, and decreased ROS, suggesting protective effects. Hence, P. clementis extract has promising neuroprotective therapeutic potential.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s13205-021-02889-3.
