A novel approach for synthesis of silver nanoparticles using Pila virens shell and its mosquito larvicidal activity

Mosquito act as a vector for variety of deadly diseases. In this study, larvicide activity was investigated in relation to Aedes aegypti (A. aegypti) and Culex quinquefasciatus (C. quinquefasciatus) of synthesised silver nanoparticles (AgNPs) of the Pila virens (P.virens) shell extract. The characterization techniques UV-vis spectral, Fourier transforms infrared spectroscopy (FTIR),High Resonance Scanning electron microscope (HR-SEM) analysis, X-ray diffraction studies (XRD), High Resonance-Transmission electron microscopy (HR-TEM) used to characterize biosynthesized AgNPs. UV-vis, absorption showed peaks of 450 nm for the biosynthesised AgNPs, SEM observed spherical shaped particles of 25.9-28.9 nm in size and the XRD pattern shows the synthesized AgNPs fcc structure. FTIR investigation shown that the esters, carboxylic acid and ether as functional groups have been intricate in the reduction of metal ions. The larvicidal efficacy of synthesized AgNPs towards a larvae of A. aegypti LC₅₀and LC₉₀ value of (37.87 and 132.86 ppm) and C. quinquefasciatus was (14.70 and 28.96 ppm) respectively. The synthesized AgNPs of P. virens confirmed highest mortality towards larvae of and A. aegypti and C. quinquefasciatus.