Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hemicholinium-3 binding: correlation with high-affinity choline uptake during changes in cholinergic activity.

A C Swann1, L O Hewitt

  • 1Department of Psychiatry, University of Texas Medical School, Houston.

Neuropharmacology
|June 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Understanding traumatic injuries and suicide risk: A conceptual exploration for the emergency department.

Journal of affective disorders·2025
Same author

Emotional self-regulation, impulsivity, 5-HTTLPR and tobacco use behavior among psychiatric inpatients.

Journal of affective disorders·2022
Same author

Illness-course modulates suicidality-related prefrontal gray matter reduction in women with bipolar disorder.

Acta psychiatrica Scandinavica·2014
Same author

Psychostimulants given in adolescence modulate their effects in adulthood using the open field and the wheel-running assays.

Brain research bulletin·2010
Same author

Interactions between bipolar disorder and antisocial personality disorder in trait impulsivity and severity of illness.

Acta psychiatrica Scandinavica·2010
Same author

Bilateral six-hydroxydopamine administration to PFC prevents the expression of behavioral sensitization to methylphenidate.

Brain research·2009

Hemicholinium-3 binding reflects presynaptic cholinergic regulation, correlating with choline uptake. This offers a valuable index for studies where choline uptake measurement is challenging, such as post-mortem research.

Area of Science:

  • Neuroscience
  • Pharmacology
  • Neurochemistry

Background:

  • Presynaptic cholinergic regulation is crucial for neurotransmission.
  • Hemicholinium-3 binding is a potential marker for cholinergic activity.
  • Understanding its utility requires comparison with established measures like choline uptake.

Purpose of the Study:

  • To evaluate hemicholinium-3 binding as an index of presynaptic cholinergic regulation.
  • To assess the correlation between hemicholinium-3 binding and high-affinity choline uptake.
  • To investigate the effects of cholinergic agonists and antagonists on these parameters.

Main Methods:

  • Measurement of hemicholinium-3 binding in rat brain regions (cerebral cortex, hippocampus, striatum).
  • Assessment of muscarinic receptor binding and high-affinity choline uptake in the same tissues.

Related Experiment Videos

  • Administration of cholinergic agonists (physostigmine, arecoline) and an antagonist (atropine).

    • Main Results:

    • Subacute treatment with physostigmine or arecoline decreased hemicholinium-3 binding, muscarinic receptor binding, and choline uptake.
    • Atropine treatment increased all three measured parameters.
    • Hemicholinium-3 binding showed a significant positive correlation with high-affinity choline uptake.

    Conclusions:

    • Hemicholinium-3 binding serves as a useful reflection of presynaptic cholinergic regulation.
    • This method is particularly valuable when direct choline uptake measurement is not feasible.
    • Applications include autoradiographic and post-mortem studies of the cholinergic system.