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Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications
Published on: September 15, 2017
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Photodegradable avidin-biotinylated polymer conjugate hydrogels for cell manipulation
Satoshi Yamaguchi1,2, Noriyuki Ohashi3, Kosuke Minamihata4
1Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan. yamaguchi@bioorg.rcast.u-tokyo.ac.jp.
Biomaterials Science
|July 1, 2021
Summary
Researchers developed novel photo-responsive protein-polymer hybrid hydrogels for advanced cell culture and sorting. These materials enable light-controlled cell recovery and selective cell liberation, offering new tools for cell engineering and artificial tissue fabrication.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Cell Biology
Background:
- Protein-synthetic polymer hybrid hydrogels offer 3D cell culture potential.
- Photo-responsive hydrogels allow spatio-temporal control of cell functions.
- Combining these properties in hybrid hydrogels is novel for cell and tissue fabrication.
Purpose of the Study:
- To develop a photo-responsive, photodegradable hydrogel for cell culture and sorting.
- To demonstrate controlled cell adhesion, survival, and recovery using light.
- To achieve selective cell liberation from a mixed-cell hydrogel matrix.
Main Methods:
- Synthesized a hydrogel using avidin and biotinylated photocleavable polyethylene glycol (PEG).
- Cultured cytokine-dependent immunocytes and fibroblasts within the hydrogel matrix.
- Utilized light irradiation to induce hydrogel degradation and cell recovery.
- Demonstrated selective cell sorting based on light-induced degradation.
Main Results:
- Successfully cultured cytokine-dependent immunocytes and controlled fibroblast adhesion/survival.
- Achieved cell recovery without damage via light-induced hydrogel degradation.
- Demonstrated selective liberation of fluorescently labeled cells from a mixed population.
- Hydrogels showed ease of preparation and functionalization with biomolecules.
Conclusions:
- Developed a versatile, photo-responsive hydrogel for cell engineering and sorting.
- Light-induced degradation enables controlled cell release and manipulation.
- The hybrid hydrogel serves as a valuable tool for cell fabrication processes.
