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Updated: Oct 31, 2025

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Biochemical Measurement of Neonatal Hypoxia
Published on: August 24, 2011
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Modification of a spectrophotometric method for assessment of monoamine oxidase activity with
Polina K Vinel1, S I Grobovoy1, A I Sinitskii1
1South Ural State Medical University, 64 Vorovskogo st., 454092, Chelyabinsk, Russia.
Analytical Biochemistry
|July 1, 2021
Abstract:
The aim of the study was to modify a simple and widely used spectrophotometric assay for MAO activity evaluation with 2,4-dinitrophenylhydrazine. A modified procedure includes molar absorption coefficients of 2,4-DNP-hydrazone benzaldehyde and 2,4-DNP-hydrazone 5-hydroxyindolylacetaldehyde as 2.3 × 104mol-1l cm-1 and 1.0 × 104 mol-1l cm-1, respectively. Such an approach allows to express specific enzyme activity as nmol product formed/min/mg protein.

