Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:24

DNA Isolation

42.9K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
42.9K
DNA Isolation01:34

DNA Isolation

195.7K
DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
195.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phytochemical profiling and system-level mechanistic insights into the anti-arthritic activity of Barleria cristata: Evidence from in-silico network pharmacology, molecular docking, in-vitro and in-vivo studies.

Journal of ethnopharmacology·2026
Same author

Efficacy of nonantimicrobial drugs against <i>Enterococcus faecalis</i> using confocal laser scanning microscopy - An <i>in vitro</i> study.

Journal of conservative dentistry and endodontics·2026
Same author

Identification of prospective PETases across prokaryotes using an in silico approach.

BMC biology·2026
Same author

Effect of <i>Moringa oleifera</i> Protein-Coated Gold Nanoparticles as Naturally Derived Disruptors of Biofilms.

International journal of biomaterials·2026
Same author

Microwave-assisted single-step synthesis of cyclic and acyclic β-aminosulfones and evaluation of their antifungal activity targeting CYP51.

RSC medicinal chemistry·2026
Same author

Crateva magna (Lour.) DC ameliorates rheumatoid arthritis via TNF-signalling pathways: An integration of in-silico, in-vitro and in-vivo approach.

Journal of ethnopharmacology·2025

Related Experiment Video

Updated: Oct 31, 2025

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains
06:18

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Published on: November 30, 2021

4.5K

Efficacy of a novel bone preprocessing method for better DNA yield.

Subhasish Sahoo1, Rashmita Samal2, Sumit Biswas3

  • 1DNA Profiling Unit, State Forensic Science Laboratory, Rasulgarh, Bhubaneswar 751010, Odisha, India; ViStA Lab, Department of Biological Sciences, BITS, Pilani - K K Birla Goa Campus, Goa 403726, India.

Forensic Science International
|July 1, 2021
PubMed
Summary

A new DNA extraction method using bone scrapping offers a 93% success rate, significantly outperforming the traditional grinding method (28% success) for forensic identification and ancient DNA analysis.

Keywords:
BoneHuman identificationSTR profilingScrapping method

More Related Videos

Author Spotlight: Exploring Advanced Therapeutic Targets in Osteosarcoma Through Spatial Transcriptomics
07:43

Author Spotlight: Exploring Advanced Therapeutic Targets in Osteosarcoma Through Spatial Transcriptomics

Published on: May 3, 2024

3.6K
A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation
10:04

A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation

Published on: July 6, 2017

9.0K

Related Experiment Videos

Last Updated: Oct 31, 2025

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains
06:18

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Published on: November 30, 2021

4.5K
Author Spotlight: Exploring Advanced Therapeutic Targets in Osteosarcoma Through Spatial Transcriptomics
07:43

Author Spotlight: Exploring Advanced Therapeutic Targets in Osteosarcoma Through Spatial Transcriptomics

Published on: May 3, 2024

3.6K
A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation
10:04

A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation

Published on: July 6, 2017

9.0K

Area of Science:

  • Forensic Science
  • Molecular Biology
  • Genetics

Background:

  • Bone and teeth are crucial biological materials for DNA profiling in missing persons, mass disasters, and ancient DNA studies.
  • Traditional DNA extraction from these samples often involves destructive grinding methods.
  • These standard methods can be inefficient and have low success rates with challenging samples.

Purpose of the Study:

  • To develop and evaluate a highly efficient DNA extraction protocol for bone samples.
  • To compare the efficacy of a novel scrapping method against the conventional grinding technique.
  • To improve DNA recovery rates for forensic and ancient DNA investigations.

Main Methods:

  • A novel DNA extraction protocol utilizing a bone scrapping technique was developed.
  • The study analyzed 25 bone samples using both the scrapping and grinding methods.
  • DNA quantity and extraction success rates were compared between the two methods.

Main Results:

  • The scrapping method yielded DNA concentrations up to 1.131 ng/µl with a 93% success rate.
  • The grinding method resulted in a significantly lower yield of 0.359 ng/µl DNA with only a 28% success rate.
  • The scrapping method demonstrated superior performance, especially for difficult-to-profile samples.

Conclusions:

  • Bone DNA extraction via scrapping is a highly efficient and successful method.
  • This technique offers a significant improvement over traditional grinding for forensic applications.
  • The scrapping method provides a valuable tool for challenging DNA profiling cases.