Prediction of protein ubiquitination sites via multi-view features based on eXtreme gradient boosting classifier

Ubiquitination is a common and reversible post-translational protein modification that regulates apoptosis and plays an important role in protein degradation and cell diseases. However, experimental identification of protein ubiquitination sites is usually time-consuming and labor-intensive, so it is necessary to establish effective predictors. In this study, we propose a ubiquitination sites prediction method based on multi-view features, namely UbiSite-XGBoost. Firstly, we use seven single-view features encoding methods to convert protein sequence fragments into digital information. Secondly, the least absolute shrinkage and selection operator (LASSO) is applied to remove the redundant information and get the optimal feature subsets. Finally, these features are inputted into the eXtreme gradient boosting (XGBoost) classifier to predict ubiquitination sites. Five-fold cross-validation shows that the AUC values of Set1-Set6 datasets are 0.8258, 0.7592, 0.7853, 0.8345, 0.8979 and 0.8901, respectively. The synthetic minority oversampling technique (SMOTE) is employed in Set4-Set6 unbalanced datasets, and the AUC values are 0.9777, 0.9782 and 0.9860, respectively. In addition, we have constructed three independent test datasets which the AUC values are 0.8007, 0.6897 and 0.7280, respectively. The results show that the proposed method UbiSite-XGBoost is superior to other ubiquitination prediction methods and it provides new guidance for the identification of ubiquitination sites. The source code and all datasets are available at https://github.com/QUST-AIBBDRC/UbiSite-XGBoost/.