Six New Diterpene Glycosides from the Soft Coral Lemnaliabournei

A chemical study on the extracts of soft coral Lemnalia bournei resulted in the isolation and identification of six new bicyclic diterpene glycosides including three new lemnaboursides E-G (1-3), and three new lemnadiolboursides A-C (4-6), along with three known lemnaboursides (7-9). Their structures were elucidated by detailed spectroscopic analysis, ECD analysis, chemical methods, and comparison with the literature data. Lemnadiolboursides A-C (4-6) contained a lemnal-1(10)-ene-7,12-diol moiety compared with the lemnaboursides. All these compounds were evaluated for antibacterial activity; cell growth inhibition of A549, Hela, HepG2, and CCRF-CEM cancer cell lines; and inhibition of LPS-induced NO production in RAW264.7 macrophages. The results indicated that compounds 1, 2, and 4-6 exhibited antibacterial activity against Staphylococcus aureus and Bacillus subtilis (MIC 4-16 μg/mL); compounds 1-9 displayed low cytotoxicity on the CCRF-CEM cell lines (IC₅₀ 10.44-27.40 µM); and compounds 1, 2, and 5 showed weak inhibition against LPS-induced NO production (IC₅₀ 21.56-28.06 μM).