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Related Experiment Video

Updated: Oct 31, 2025

Author Spotlight: A Novel Cell Injection Method with Minimum Invasion

Author Spotlight: A Novel Cell Injection Method with Minimum Invasion

Published on: April 21, 2023

Novel Techniques to Improve Precise Cell Injection.

Walter Linzenbold¹, Andreas Fech¹, Manuela Hofmann¹

¹Erbe Elektromedizin GmbH, 72072 Tuebingen, Germany.

International Journal of Molecular Sciences

|July 2, 2021

Summary

A new needle-free water-jet system precisely injects cells into urethral sphincter tissue. This minimally invasive technique improves cell delivery accuracy and viability for regenerative medicine applications.

Keywords:

cell therapy medical technology regenerative medicine stress urinary incontinence tissue engineering water-jet technology

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Last Updated: Oct 31, 2025

Author Spotlight: A Novel Cell Injection Method with Minimum Invasion

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Published on: February 26, 2017

Area of Science:

Biomedical Engineering
Regenerative Medicine
Minimally Invasive Surgery

Background:

Conventional needle injections into the urethral sphincter muscle have limitations, with approximately 50% of animals showing imprecise cell deposition.
Shotgun injection approaches increase tissue injury risk due to multiple circumferential insertions.

Purpose of the Study:

To develop and evaluate a novel needle-free water-jet injection system for precise cell delivery.
To assess the system's applicability in urethral sphincter tissue and other tissues for minimally invasive procedures.

Main Methods:

A novel water-jet injection system designed for endoscope/cystoscope working channels was developed.
Key parameters analyzed included injector design, pressure ranges for tissue penetration, and injection media composition.
Cell viability post-injection was assessed under various settings.

Main Results:

The water-jet system demonstrated precise cell injection capabilities in urethral sphincter tissue.
Optimized settings were identified to ensure high cell viability after injection.
The method effectively delivered cells to both superficial and deeper tissue layers.

Conclusions:

The needle-free water-jet system offers a precise and minimally invasive alternative for cell injection.
This technology has broad applications in regenerative medicine and tissue engineering, particularly for targeted therapies.
The system's ability to deliver cells to various depths enhances its potential for complex tissue targets like the urethral sphincter.