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Related Experiment Videos

Multiple elements are required for expression of an intermediate filament gene.

C M Sax1, F X Farrell, J A Tobian

  • 1Department of Human Genetics, Massey Cancer Center, Richmond, VA 23298.

Nucleic Acids Research
|August 25, 1988
PubMed
Summary

Vimentin gene expression is regulated by multiple cis-acting elements, including Sp1 transcription factor binding sites. A fibroblast-specific enhancer element was identified, contributing to vimentin

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Gene Regulation

Background:

  • Vimentin, an intermediate filament protein, exhibits unique gene expression patterns.
  • Its 5'-flanking region contains GC boxes, known binding sites for the Sp1 transcription factor.
  • Understanding vimentin gene regulation is crucial for comprehending cellular processes.

Purpose of the Study:

  • To investigate the regulatory mechanisms controlling vimentin gene expression.
  • To identify specific cis-acting elements and transcription factors involved in vimentin regulation.
  • To compare vimentin gene activity in cells with high versus no expression.

Main Methods:

  • Analysis of vimentin 5'-end:CAT fusions in mouse L cells (high expression) and MH1C1 cells (no expression).

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  • Microinjection of constructs into Xenopus oocytes to assess transcriptional activity.
  • In vivo expression studies and in vitro DNA-binding assays to implicate Sp1.

    • Main Results:

    • Sp1 is implicated as a general regulatory factor in vimentin gene expression through in vivo and in vitro studies.
    • A fibroblast-specific enhancer element located between -321 and -160 significantly increased CAT activity in mouse L cells.
    • Low transcriptional activity in MH1C1 cells may result from the absence of positive factors or the presence of repressor elements.

    Conclusions:

    • Vimentin gene expression is controlled by multiple cis-acting elements.
    • Sp1 plays a significant role in the regulation of vimentin.
    • A fibroblast-specific enhancer contributes to the unique expression pattern of vimentin.