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Updated: Oct 31, 2025

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Published on: February 16, 2017

Optimal Halbach Configuration for Flow-through Immunomagnetic CTC Enrichment.

Michiel Stevens¹, Peng Liu^1,2, Tom Niessink¹

¹Department of Medical Cell BioPhysics, University of Twente, 7522 NB Enschede, The Netherlands.

Diagnostics (Basel, Switzerland)

|July 2, 2021

Summary

Optimizing immunomagnetic enrichment for circulating tumor cells (CTCs) using flow-through magnetophoresis enhances sample processing. This method improves the isolation and enumeration of CTCs from larger blood volumes for personalized cancer therapy.

Keywords:

Halbach circulating tumor cell enrichment finite element model flow magnetic

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Preparation of Myeloid Derived Suppressor Cells MDSC from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting AutoMACS

Published on: June 18, 2012

Area of Science:

Biomedical Engineering
Oncology
Cell Biology

Background:

Standard methods for circulating tumor cell (CTC) analysis are limited by low cell frequency and small blood volumes.
Current techniques struggle to consistently measure treatment effects or guide personalized therapy due to insufficient CTC yield.
Diagnostic leukapheresis increases sample size but requires advanced processing methods.

Purpose of the Study:

To optimize immunomagnetic enrichment for processing larger sample volumes.
To develop and validate a flow-through magnetophoretic system for enhanced CTC isolation.
To improve the efficiency and reliability of CTC enumeration for clinical applications.

Main Methods:

Optimization of an immunomagnetic enrichment process using a flow-through magnetophoretic system.
Modeling and calculation of optimal Halbach array configurations for magnetic separation.
Experimental testing of magnetic arrays with anti-EpCAM ferrofluid and cell lines expressing varying EpCAM levels.
Comparison of predicted recoveries with experimentally measured cell distributions.

Main Results:

The study presents an optimized flow-through magnetophoretic system for immunomagnetic enrichment.
Calculated optimal Halbach array dimensions were validated experimentally.
Predicted cell recoveries aligned with experimental data within measurement uncertainty.
The developed method demonstrated effective separation of cells based on EpCAM expression.

Conclusions:

The optimized magnetophoretic system enables efficient processing of larger sample volumes for CTC analysis.
This approach can improve CTC enumeration and isolation for monitoring treatment response and personalizing therapy.
The methodology is adaptable for optimizing various magnetic separation techniques in biomedical research.