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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: Oct 31, 2025

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

Published on: July 21, 2017

Fluorescent Protein-Based Autophagy Biosensors.

Heejung Kim^1,2, Jihye Seong^1,2

¹Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Korea.

Materials (Basel, Switzerland)

|July 2, 2021

Summary

Researchers reviewed fluorescent protein biosensors for studying autophagy, a key cellular process implicated in diseases like neurodegeneration. These tools enable real-time monitoring of autophagy in live cells, aiding disease mechanism research.

Keywords:

autophagy biosensors fluorescence imaging fluorescent protein neurodegenerative diseases

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Last Updated: Oct 31, 2025

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

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Published on: July 21, 2017

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Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond

Published on: July 27, 2013

Area of Science:

Cell Biology
Biochemistry
Molecular Medicine

Background:

Autophagy is a fundamental cellular degradation process crucial for maintaining homeostasis.
Dysregulation of autophagy is linked to various pathologies, including neurodegenerative diseases.
Studying autophagy requires sophisticated biochemical, chemical, and imaging techniques.

Purpose of the Study:

To review and analyze fluorescent protein (FP)-based biosensors for studying autophagy.
To discuss the design principles, advantages, and limitations of these biosensors.
To highlight their utility in live-cell imaging and real-time monitoring of autophagy.

Main Methods:

Review of existing literature on FP-based autophagy biosensors.
Analysis of FP properties (pH-sensitivity, stability, brightness, spectral profile, FRET) for biosensor design.
Discussion of imaging techniques for autophagy monitoring.

Main Results:

FP-based biosensors offer sensitive, real-time detection of autophagy progression in live cells.
These biosensors provide high spatiotemporal resolution for observing autophagy dynamics.
Consideration of FP physicochemical properties is key to optimizing biosensor performance.

Conclusions:

FP-based biosensors are powerful tools for investigating autophagy mechanisms.
Advanced optobiochemical strategies show promise for future autophagy research.
Improved understanding of autophagy through these technologies can advance treatments for related diseases.