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Molecular Characterization of KPC-2-Producing Enterobacter cloacae Complex Isolates from Cali, Colombia
Aura Falco1, Daniela Guerrero1, Isabella García1
1Grupo de Investigación en Microbiología, Industria y Ambiente (GIMIA), Facultad de Ciencias Básicas, Universidad Santiago de Cali, Cali 760035, Colombia.
Antibiotics (Basel, Switzerland)
|July 2, 2021
Summary
The Enterobacter cloacae complex, a growing threat, shows high carbapenem resistance in Colombian hospitals. Molecular analysis identified carbapenemase genes, like blaKPC-2, and diverse genetic strains, aiding surveillance strategies.
Area of Science:
- Microbiology
- Infectious Diseases
- Public Health
Background:
- The Enterobacter cloacae complex is an opportunistic pathogen causing significant public health concern due to rising carbapenem resistance.
- Carbapenem resistance diminishes the efficacy of beta-lactam antibiotics, crucial for treating Gram-negative bacterial infections.
Purpose of the Study:
- To molecularly characterize Enterobacter cloacae complex isolates resistant to cephalosporins and carbapenems.
- To identify carbapenem resistance genes and assess the genetic diversity of these isolates in Colombian hospitals.
Main Methods:
- Molecular detection of carbapenemase genes (blaKPC, blaVIM, blaNDM, blaOXA-48-like).
- Multilocus sequence typing (MLST) to determine genetic relationships among isolates.
Main Results:
- 43% of isolates harbored the blaKPC-2 gene variant.
- MLST revealed high genetic diversity, with Sequence Type 510 (ST510) being the most prevalent (50%).
Conclusions:
- Identification of carbapenem resistance genes and prevalent genotypes is crucial for developing effective prevention and surveillance strategies.
- This study provides essential data for combating Enterobacter cloacae complex infections in Colombian healthcare settings.
