[Spontaneous DNA damage and DNA polynucleotide ligase activity in thymocyte populations differing in

T M Alferova, V A Tronov, V A Soldatenkov

Radiobiologiia

|July 1, 1988

Abstract:

Single-strand breaks (SB) in DNA of irradiated thymocytes are first repaired, at a half-recovery period of a few minutes, then the number of breaks rapidly increases indicating that in addition to chromatin endonucleolysis there is an imbalance between the repair enzymes that generate DNA breaks and eliminate them. The rate of SB accumulation in thymocyte fractions, upon incubation of the cells with 1-beta-D-arabinofuranosyl cytosine (AraC) and hydroxyurea (HU), is directly proportional to their radiosensitivity, whereas DNA ligase activity is higher in a fraction of highly radioresistant thymocytes. Chemical compounds that increase the survival rate of irradiated thymocytes decelerate SB accumulation in DNA and sensitivity of cells to AraC and HU. Ineffective substances have no such an effect.

Related Experiment Videos

[Spontaneous DNA damage and DNA polynucleotide ligase activity in thymocyte populations differing in

Related Concept Videos

[Radiation preconditioning of mouse retina results in tolerance to MNU-induced degeneration and stimulates retinal recovery].

[Damage and functional recovery of the mouse retina after exposure to ionizing radiation and methylnitrosourea].

[Mechanisms of radioresistance in terminally differentiated cells of mature retina].

[Effectiveness of DNA repair and expression of MLH1, MSH2 and FASR in lymphocytes of patients with chemotherapy-responsive, disseminated cutaneous melanoma].

[Cellular markers based on DNA damage and repair (BER, MMR), expression of MLHI, MSH2, FasR, and cell death of lymphocytes as predictive parameters for clinical response to chemotherapy of melanoma].

[Genetic markers of melanoma].

[The postradiation effect of noradrenaline, serotonin and dopamine on the activity of the Na-K pump in rat brain slices].

[The ratio of the contributions from the direct and indirect actions of UV radiation in changing the functional properties of the complement system].

[The biological action of nuclear fission products. The late sequelae of the lesions].

[The effect of hematoporphyrin derivative on the postradiation recovery of hemopoiesis in mice].

[The protective action of a natural preparation of anthocyan (pelargonidin-3,5-diglucoside)].

[An evaluation of the radioprotective action of antioxidants on normal human cells and in genomic diseases by using different cytogenetic tests].

Protective Effects of Fushiming Against Microwave Radiation-Induced Retinal Damage: A Network Pharmacology and Experimental Study.

C7 Vertebral Pathological Compressive Fracture after Radiotherapy for Esophageal Cancer: A Case Report.

Multi-Sequence Fusion MRI Deep Learning Models for Discrimination of Recurrent Nasopharyngeal Carcinoma and Osteoradionecrosis.

Disseminated Nocardia farcinica Infection Presenting as Multiple Intramuscular Abscesses Mimicking Skeletal Muscle Metastases in a Patient With Hepatocellular Carcinoma.

Related Experiment Videos

[Spontaneous DNA damage and DNA polynucleotide ligase activity in thymocyte populations differing in...

Abstract:

Related Concept Videos

Related Articles

[Spontaneous DNA damage and DNA polynucleotide ligase activity in thymocyte populations differing in