Related Experiment Video
Updated: Oct 30, 2025

Reconstitution of Actin-Based Motility with Commercially Available Proteins
Published on: October 28, 2022
Fe65: A Scaffolding Protein of Actin Regulators
Vanessa Augustin1, Stefan Kins1
1Division of Human Biology and Human Genetics, Technical University of Kaiserslautern, 67663 Kaiserslautern, Germany.
Abstract:
The scaffolding protein family Fe65, composed of Fe65, Fe65L1, and Fe65L2, was identified as an interaction partner of the amyloid precursor protein (APP), which plays a key function in Alzheimer's disease. All three Fe65 family members possess three highly conserved interaction domains, forming complexes with diverse binding partners that can be assigned to different cellular functions, such as transactivation of genes in the nucleus, modulation of calcium homeostasis and lipid metabolism, and regulation of the actin cytoskeleton. In this article, we rule out putative new intracellular signaling mechanisms of the APP-interacting protein Fe65 in the regulation of actin cytoskeleton dynamics in the context of various neuronal functions, such as cell migration, neurite outgrowth, and synaptic plasticity.
Related Concept Videos
Formation of Higher-order Actin Filaments
The high-order actin...
Generation of Straight or Branched Actin Filaments
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
Introduction to Actin
Actin Filament Depolymerization
In F-actin, the ADF/cofilin proteins...
Actin Polymerization and Cell Motility
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
Cytoskeletal Accessory Proteins

