Paper-Based Immunosensors with Bio-Chemiluminescence Detection

Area of Science:

• Analytical Chemistry
• Biomedical Engineering
• Diagnostics

Background:

• Paper-based analytical devices (PADs) have evolved significantly for point-of-care (POC) diagnostics.
• Chemiluminescent (CL) immunoassays adapted to paper formats show great potential for portable, on-site applications.
• PADs combined with CL detection offer a unique solution for POC testing.

Purpose of the Study:

• To review the progress and challenges in developing paper-based diagnostic devices.
• To identify the barriers preventing the widespread clinical adoption of these technologies.
• To highlight areas for future research to enable routine application of PADs.

Main Methods:

• Literature review of advancements in paper-based analytical devices.
• Analysis of chemiluminescent immunoassays integrated with paper platforms.
• Evaluation of factors limiting the translation of research into clinical practice.

Main Results:

• Significant advancements have been made in adapting analytical methods, particularly CL immunoassays, to paper substrates.
• Despite scientific progress, a gap exists between research output and the real-world implementation of these devices.
• Key issues hindering routine use include scalability, standardization, and integration into existing healthcare workflows.

Conclusions:

• Paper-based diagnostics, especially CL immunoassays, are well-suited for POC applications.
• Overcoming current limitations requires targeted research and development efforts.
• Future work should focus on addressing practical challenges to facilitate routine clinical use of these innovative diagnostic tools.