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Updated: Oct 30, 2025
04:23
A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease
Published on: April 28, 2019
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Dengue and the Lectin Pathway of the Complement System
Romchat Kraivong1,2, Nuntaya Punyadee2,3, M Kathryn Liszewski4
1Molecular Biology of Dengue and Flaviviruses Research Team, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Bangkok 12120, Thailand.
Viruses
|July 2, 2021
Summary
The dengue virus (DENV) uses its surface N-glycans to evade immune responses. Mannan-binding lectin (MBL) neutralizes DENV, but the virus counteracts this through secreted nonstructural protein 1 (sNS1).
Area of Science:
- Immunology
- Virology
- Infectious Diseases
Background:
- Dengue virus (DENV) infection poses a global health and economic challenge, with severity influenced by serotype and prior exposure.
- The complement system, particularly the lectin pathway (LP), plays a dual role in dengue pathogenesis and protection.
- Foreign glycan recognition by lectin pattern recognition receptors (PRRs) is crucial for LP activation.
Purpose of the Study:
- To investigate the role of N-glycans on the DENV envelope in modulating lectin pathway activation and immune evasion.
- To elucidate the mechanisms by which mannan-binding lectin (MBL) neutralizes DENV and how DENV counteracts these effects.
Main Methods:
- Analysis of N-glycan structures on DENV envelope proteins.
- Assessment of MBL binding efficiency to different DENV glycoforms.
- Evaluation of complement activation-dependent and -independent neutralization mechanisms.
- Investigation of the role of DENV secreted nonstructural protein 1 (sNS1) in immune evasion.
Main Results:
- DENV N-glycans influence MBL binding and subsequent lectin pathway activation.
- MBL neutralizes DENV through direct glycan recognition and complement-mediated opsonization.
- DENV sNS1 antagonizes MBL activity, promoting viral survival and dissemination.
- Serum concentrations and polymorphisms of lectin PRRs impact LP-mediated dengue immunity.
Conclusions:
- DENV N-glycan variations are key determinants of host immune interactions.
- MBL-mediated neutralization is a critical host defense mechanism against DENV.
- DENV sNS1 represents a significant viral evasion strategy, highlighting a complex interplay between virus and host immunity.
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