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Conjugated Proteins

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Published on: March 1, 2019

Heparan Sulfate Binding Cationic Peptides Restrict SARS-CoV-2 Entry.

Rahul K Suryawanshi¹, Chandrashekhar D Patil¹, Raghuram Koganti¹

¹Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL 60612, USA.

Pathogens (Basel, Switzerland)

|July 2, 2021

Summary

Cationic peptides G1 and G2 show potential in inhibiting SARS-CoV-2 entry by targeting ACE2 and heparan sulfate, crucial for virus cell attachment and infection spread.

Keywords:

SARS-CoV-2 cationic peptide entry inhibitors heparan sulfate pseudotyped virus

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A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation

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Published on: January 9, 2019

Area of Science:

Virology
Biochemistry
Drug Discovery

Background:

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic necessitates novel therapeutic strategies.
Understanding SARS-CoV-2 entry mechanisms is key to developing effective inhibitors.
Cellular receptors, including angiotensin-converting enzyme 2 (ACE2) and heparan sulfate (HS), are critical for viral entry.

Purpose of the Study:

To evaluate the efficacy of cationic peptides G1 and G2 as SARS-CoV-2 entry inhibitors.
To investigate the interaction of G1 and G2 peptides with HS and ACE2.
To assess the impact of G1 and G2 peptides on cell surface HS levels.

Main Methods:

In silico binding affinity studies to predict peptide-receptor interactions.
Experimental evaluation of prophylactic treatment with G1 and G2 peptides.
Assessment of cell surface HS levels following peptide treatment.

Main Results:

In silico analysis indicated potential binding sites for G1 and G2 peptides on HS and ACE2.
Prophylactic treatment with G1 and G2 peptides reduced cell surface HS.
These findings suggest dual mechanisms of virus entry inhibition.

Conclusions:

Cationic peptides G1 and G2 demonstrate potential for inhibiting SARS-CoV-2 entry.
The peptides interfere with virus attachment by targeting HS and ACE2.
Cationic peptides represent a promising avenue for developing SARS-CoV-2 entry inhibitors.