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Related Concept Videos

Elastin is Responsible for Tissue Elasticity01:12

Elastin is Responsible for Tissue Elasticity

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Elastic fiber contains the protein elastin along with lesser amounts of other proteins and glycoproteins. The main property of elastin is that it will return to its original shape after being stretched or compressed. Elastic fibers are prominent in elastic tissues found in skin and the elastic ligaments of the vertebral column.
Ligaments and tendons are made of dense regular connective tissue, but in ligaments not all fibers are parallel. Dense regular elastic tissue contains elastin fibers and...
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Elastin-Plasma Hybrid Hydrogels for Skin Tissue Engineering.

Marija Stojic1,2, Joaquín Ródenas-Rochina3, María Luisa López-Donaire2

  • 1CEITEC-Central European Institute of Technology, Brno University of Technology, Purkyňova 123, 61200 Brno, Czech Republic.

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Summary

This study enhances fibrin hydrogels for skin engineering by incorporating elastin-like recombinamers (ELR). The modified hydrogels show improved mechanical properties and elasticity, with potential for pharmaceutical and cosmetic testing.

Keywords:
bilayered in vitro skin substitutesbioengineered skinelastin like recombinamersfibrin hydrogelshuman plasma-derived fibrin hydrogelshybrid plasma-elastin hydrogelsskin tissue engineering

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Polymer Chemistry

Background:

  • Plasma-derived fibrin hydrogels are widely used in skin engineering but suffer from poor mechanical properties, rapid degradation, and contraction.
  • These limitations hinder reproducibility and the lifespan of engineered skin constructs.
  • Improving mechanical strength, elasticity, and biological performance is crucial for advanced applications.

Purpose of the Study:

  • To enhance plasma-derived fibrin hydrogels by incorporating an elastin-like recombinamer (ELR) network.
  • To evaluate the impact of ELR incorporation on hydrogel properties, including mechanical strength, elasticity, degradation, and cell proliferation.
  • To explore the potential of these hybrid hydrogels for applications in skin testing platforms.

Main Methods:

  • Incorporation of two types of ELR, modified with azide (SKS-N₃) and cyclooctyne (SKS-Cyclo) groups, into fibrin hydrogels at a 1:1 molar ratio.
  • Testing of three different serine-lysine-serine (SKS) sequence concentrations (1, 3, and 5 wt.%).
  • Assessment of gelation time, contraction, mechanical properties, elasticity, and proliferation of human primary fibroblasts (hFBs) and keratinocytes (hKCs).

Main Results:

  • Reduced gelation time and contraction observed with increasing SKS content.
  • Enhanced mechanical properties and elasticity were achieved at SKS concentrations of 3% and 5 wt.%.
  • Optimal hFB proliferation occurred at 1 wt.% SKS, while hKC proliferation increased at 5 wt.% SKS.

Conclusions:

  • Hybrid fibrin-ELR hydrogels demonstrate improved mechanical characteristics and reduced contraction compared to native fibrin hydrogels.
  • A balance between mechanical enhancement and biological response is necessary, suggesting tailored ELR concentrations for specific applications.
  • These advanced hydrogels show significant potential as platforms for pharmaceutical and cosmetic product testing.