Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.

Structural Protein Function

Structural Protein Function

Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...

Notch Signaling Pathway

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...

Negative Regulator Molecules

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.

Canonical Wnt Signaling Pathway

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...

The Intrinsic Apoptotic Pathway

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Impact of BECLIN1 haploinsufficiency on goblet cell function and susceptibility to colitis.

Cell death & disease·2026

Same author

BECLIN-1/BECN1 at the barrier: a gatekeeper of epithelial and endothelial homeostasis.

Autophagy reports·2025

Same author

Author Correction: In situ visualization of endothelial cell-derived extracellular vesicle formation in steady state and malignant conditions.

Nature communications·2025

Same author

Computational design of potent and selective binders of BAK and BAX.

Science advances·2025

Same author

Development of a Novel Biomarker Platform for Profiling Key Protein-Protein Interactions to Predict the Efficacy of BH3-Mimetic Drugs.

Cancers·2025

Same author

BECLIN-1 is essential for the maintenance of gastrointestinal epithelial integrity by regulating endocytic trafficking, F-actin organization, and lysosomal function.

Autophagy reports·2025

Search research articles

Related Experiment Video

Updated: Oct 30, 2025

Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies

Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies

Published on: March 19, 2014

BECLIN1: Protein Structure, Function and Regulation.

Sharon Tran^1,2, W Douglas Fairlie^1,2,3, Erinna F Lee^1,2,3

¹Olivia Newton-John Cancer Research Institute, Heidelberg, VIC 3084, Australia.

|July 2, 2021

Summary

BECLIN1 regulates autophagy and membrane trafficking by forming PI3K complexes. Its structure, interactions, and properties are key to these essential cellular processes and organismal health.

Keywords:

BCL-2 BECLIN1 PI3K Class III complexes autophagy

More Related Videos

Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4',6-Diamidino-2-phenylindole Staining in Caenorhabditis elegans

Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4',6-Diamidino-2-phenylindole Staining in Caenorhabditis elegans

Published on: July 30, 2018

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

Related Experiment Videos

Last Updated: Oct 30, 2025

Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies

Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies

Published on: March 19, 2014

Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4',6-Diamidino-2-phenylindole Staining in Caenorhabditis elegans

Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4',6-Diamidino-2-phenylindole Staining in Caenorhabditis elegans

Published on: July 30, 2018

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

Area of Science:

Cell Biology
Molecular Biology
Biochemistry

Background:

BECLIN1 is a critical regulator of autophagy, a fundamental process for cell survival.
It participates in Class III Phosphoinositide 3-Kinase (PI3K) complexes, producing essential lipids for autophagy and membrane trafficking.

Purpose of the Study:

To review the structural, biophysical, and biochemical properties of BECLIN1.
To explore how BECLIN1's protein interactions modulate its function.
To discuss the impact of BECLIN1 on organismal physiology.

Main Methods:

Literature review of existing studies on BECLIN1.
Analysis of structural, biophysical, and biochemical data.
Examination of BECLIN1's protein interactome.

Main Results:

BECLIN1's properties elucidate its allosteric regulation of autophagy and membrane trafficking.
The diverse protein interactome of BECLIN1 significantly influences its regulatory role.
BECLIN1 plays a crucial role in various aspects of organismal physiology.

Conclusions:

Understanding BECLIN1's properties and interactions is vital for comprehending autophagy and membrane trafficking.
BECLIN1's function has broad implications for mammalian survival and overall physiology.