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Related Concept Videos

Chronic Kidney Disease I: Introduction01:25

Chronic Kidney Disease I: Introduction

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Chronic Kidney Disease (CKD) arises when the kidneys progressively lose their ability to function, ultimately leading to end-stage renal disease. At this advanced stage, the kidneys can no longer filter waste or maintain essential body functions, requiring renal replacement therapy (RRT) through dialysis or a kidney transplant for survival.Early-stage chronic kidney disease and detection challengesIn CKD's early stages, symptoms often remain absent because healthy nephrons compensate for...
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The glomerulus and Bowman's capsule are two essential components of the nephron, which is the functional unit of the kidney. These microscopic structures play a critical role in the process of blood filtration to produce urine.
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The kidneys are intricate organs with millions of working units known as nephrons. Each nephron features two major structures: the renal corpuscle, which facilitates blood plasma filtration, and the renal tubule, which handles the glomerular filtrate. Blood supply is directly linked to the nephrons. The renal corpuscle consists of the glomerulus, a capillary network, and the Bowman's capsule, a double-walled epithelial structure that encases the glomerulus. The filtering of blood plasma...
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Acute Kidney Injury II: Pathophysiology01:29

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Acute kidney injury (AKI) causes are categorized into three primary categories based on the location of the injury: prerenal, intrarenal (or intrinsic), and postrenal causes. This classification guides clinical management and illustrates how different pathways can impair kidney function.Etiology and Pathophysiology of Acute Kidney Injury1. Prerenal causesEtiology: Prerenal Acute Kidney Injury, the most common type, occurs when reduced blood flow to the kidneys decreases filtration capacity...
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Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration01:28

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Glomerular filtration rate (GFR) can be estimated from serum creatinine using the modification of diet in renal disease (MDRD) formula or the chronic kidney disease–epidemiology collaboration (CKD–EPI) equation. Both methods are widely used in clinical practice to assess kidney function and guide treatment decisions.The MDRD equation does not require weight or height measurements and is normalized to the body surface area of 1.73 m², considered the average adult surface area.
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Chronic Kidney Disease III: Interprofessional Care

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Chronic kidney disease (CKD) requires collaborative and comprehensive management. CKD progresses through stages and can lead to end-stage kidney disease (ESKD) if untreated. Interprofessional collaboration and patient education are crucial, enabling patients to manage their health and improve their quality of life.Diagnostic approach for chronic kidney diseaseThe diagnosis of CKD primarily focuses on the glomerular filtration rate (GFR), which assesses kidney function by measuring how well...
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Related Experiment Video

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MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues
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MRTF: Basic Biology and Role in Kidney Disease.

Maria Zena Miranda1, Zsuzsanna Lichner1, Katalin Szászi1,2

  • 1Keenan Research Centre for Biomedical Science of the St. Michael's Hospital, Toronto, ON M5B 1W8, Canada.

International Journal of Molecular Sciences
|July 2, 2021
PubMed
Summary

Myocardin-related transcription factor (MRTF) regulates genes via the cytoskeleton, impacting cell functions and organ fibrosis. This review details MRTF

Keywords:
Rho GTPasesactin cytoskeletongene expressionkidney fibrosismyofibroblastnucleocytoplasmic shuttlingprofibrotic epithelial phenotypetranscription factors

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Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells
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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Rho family GTPases regulate gene expression through cytoskeletal organization.
  • Myocardin-related transcription factor (MRTF) shuttles to the nucleus upon actin polymerization.
  • The MRTF/SRF complex controls genes involved in cytoskeleton remodeling, contractility, and extracellular matrix organization.

Purpose of the Study:

  • To summarize the basic biology and regulation of MRTF.
  • To highlight MRTF's role in the pathobiology of kidney disease and fibrosis.

Main Methods:

  • Review of existing literature on MRTF function and regulation.
  • Analysis of MRTF's involvement in cellular processes and disease mechanisms.

Main Results:

  • MRTF activation by various stimuli influences cell motility, development, metabolism, metastasis, inflammation, and organ fibrosis.
  • MRTF plays a key role in inducing a profibrotic epithelial phenotype (PEP) and fibroblast-myofibroblast transition.

Conclusions:

  • MRTF is a crucial transcriptional coactivator with significant physiological and pathological relevance.
  • MRTF is implicated in the pathogenesis of chronic kidney disease and renal fibrosis through direct transcriptional and epigenetic mechanisms.