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Association of Matrix Metalloproteinases with Coronary Artery Calcification in Patients with CHD

Yana V Polonskaya1, Elena V Kashtanova1, Ivan S Murashov2

  • 1Research Institute of Internal and Preventive Medicine-Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (IIPM-Branch of IC&G SB RAS), 175/1 B. Bogatkova Str., 630089 Novosibirsk, Russia.

Journal of Personalized Medicine
|July 2, 2021
PubMed

Insights

Matrix metalloproteinases (MMPs) are linked to coronary artery calcification. MMP-9 showed a significant association with calcification risk, suggesting MMPs

Area of Science:

  • Cardiovascular Biology
  • Biochemistry
  • Medical Research

Background:

  • Coronary artery calcification (CAC) is a significant indicator of cardiovascular disease.
  • The role of matrix metalloproteinases (MMPs) in the pathogenesis of atherosclerosis and vascular calcification is not fully understood.

Purpose of the Study:

  • To investigate the association between serum and vascular levels of various matrix metalloproteinases (MMPs) and coronary artery calcification (CAC) in patients with and without coronary heart disease (CHD).
  • To explore the potential of specific MMPs as biomarkers for CHD and CAC.

Main Methods:

  • Serum and coronary artery samples were collected from 78 patients with CHD and 36 controls.
  • Serum MMP levels (MMP-1, -2, -3, -7, -9, -10, -12, -13) were measured using multiplex analysis.
  • Vascular MMP levels (MMP-1, -3, -7, -9) and MMP-9 expression were assessed via enzyme immunoassay and immunohistochemistry, respectively.
  • Associations between MMP levels, calcium content, and other biomarkers (osteoprotegerin, osteonectin, osteocalcin, osteopontin) were analyzed.

Main Results:

  • Patients with CHD exhibited elevated serum levels of MMP-1, MMP-7, and MMP-12 compared to controls.
  • Serum MMP-1 and MMP-3 levels correlated with calcium content in coronary arteries.
  • MMP-9 expression was significantly higher in calcified plaques compared to non-calcified ones.
  • MMP-9 was strongly associated with an increased relative risk of coronary artery calcification, confirmed by immunohistochemistry.

Conclusions:

  • Specific matrix metalloproteinases, particularly MMP-9, play a role in the process of coronary artery calcification.
  • MMP-1, MMP-7, and MMP-12 show potential as biomarkers for identifying coronary heart disease.

Keywords:
atherosclerosiscalcificationcoronary heart diseasemetalloproteinasesmultiplex assay

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