Temperature-Dependent Dynamical Evolution in Coum/SBE-β-CD Inclusion Complexes Revealed by Two-Dimensional FTIR...

Area of Science:

• Spectroscopy and Analytical Chemistry
• Materials Science
• Physical Chemistry

Background:

• Sulfobutylether-β-cyclodextrin (SBE-β-CD) is a host agent for poorly soluble compounds.
• Coumestrol (Coum) is an active compound with anti-viral and anti-oxidant properties.
• Understanding temperature-dependent dynamics in inclusion complexes is crucial for drug delivery systems.

Purpose of the Study:

• To investigate the temperature-dependent dynamical evolution of SBE-β-CD/Coumestrol inclusion complexes.
• To apply Fourier transform infrared spectroscopy in attenuated total reflectance geometry (FTIR-ATR) combined with 2D correlation analysis (2D-COS) for this investigation.
• To explore the potential for developing optimized temperature-sensitive drug carrier systems.

Main Methods:

• Utilized a combination of FTIR-ATR spectroscopy and 2D correlation analysis (2D-COS).
• Calculated synchronous and asynchronous 2D spectra across three wavenumber regions (960-1320 cm⁻¹, 1580-1760 cm⁻¹, 2780-3750 cm⁻¹).
• Studied the temperature range from 250 K to 340 K.

Main Results:

• The 2D-COS analysis enhanced spectral resolution, enabling the tracking of dynamical events.
• The sequential order of events involving specific functional groups within the complex was determined.
• This approach overcame limitations of conventional 1D FTIR-ATR analysis.

Conclusions:

• The combined FTIR-ATR and 2D-COS method provides detailed insights into the temperature-dependent behavior of SBE-β-CD/Coum inclusion complexes.
• The findings facilitate the identification of sequential events driven by temperature changes.
• This research lays the groundwork for designing advanced temperature-sensitive drug delivery systems.