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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Oct 30, 2025

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

An ISFET Microarray Sensor System for Detecting the DNA Base Pairing.

Peng Sun¹, Yongxin Cong¹, Ming Xu¹

¹State Key Laboratory of ASIC and System, School of Microelectronics, Fudan University, Shanghai 200433, China.

|July 2, 2021

Summary

This summary is machine-generated.

A novel semiconductor chip-based sensor array successfully detects DNA base pairing for faster, cheaper gene sequencing. This ion-sensitive field-effect transistor (ISFET) technology offers a cost-effective solution for genetic analysis and molecular biology applications.

Keywords:

DNA base pairing ISFET microarray chip high resolution microfluidic system pH measurement

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Last Updated: Oct 30, 2025

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Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Area of Science:

Biotechnology
Semiconductor Technology
Genomics

Background:

Conventional DNA sequencing is costly and complex, requiring bulky equipment and fluorescent labeling.
Advancements in semiconductor chip technology offer potential for more efficient sequencing methods.

Purpose of the Study:

To design and validate a large-scale ion-sensitive field-effect transistor (ISFET) array chip for detecting DNA base pairing.
To develop a cost-efficient and compact DNA sequencing sensor system.

Main Methods:

Designed a complementary metal-oxide semiconductor (CMOS) compatible ISFET array chip with over 13 million sensitive units.
Integrated the chip with a microfluidic system and a test platform to create a DNA base pairing detection system.
Achieved high sensor resolution (≥0.5 mV) capable of detecting 0.01 pH changes.

Main Results:

Successfully detected DNA base pairing using the developed sensor system.
Demonstrated the chip's high resolution and sensitivity for pH change detection.
Validated the potential of the ISFET array chip as a core component for a complete DNA sequencing system.

Conclusions:

The developed ISFET array chip offers a low-cost, high-resolution solution for DNA base pairing detection.
This technology paves the way for compact and efficient DNA sequencing systems.
The system has significant potential applications in molecular biology, gene diagnosis, and gene therapy.