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Identification and Study of Biomarkers from Novichok-Inhibited Butyrylcholinesterase in Human Plasma
Woo-Hyeon Jeong1, Jin-Young Lee1, Kyoung-Chan Lim1
1Agency for Defense Development (ADD), P.O. Box 35, Yuseong-gu, Daejeon 34186, Korea.
Abstract:
To identify biomarkers of ethyl (1-(diethylamino)ethylidene)phosphoramidofluoridate (A234)- or methyl (1-(diethylamino)ethylidene)phosphoramidofluoridate (A232)-inhibited butyrylcholinesterase (BChE), we investigated nonapeptide adducts containing the active site serine, which plays a key role in enzyme activity, using LC-MS/HRMS. Biomarkers were acquired as expected, and they exhibited a significant amount of fragment ions from the inhibiting agent itself, in contrast to the MS2 spectra of conventional nerve agents. These biomarkers had a higher abundance of [M+2H]2+ ions than [M+H]+ ions, making doubly charged ions more suitable for trace analysis.
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