Physiological Responses of Wheat Seedlings to Soil Waterlogging Applied after Treatment with Selective Herbicide

Waterlogging impairs crop development and considerably affects plant productivity worldwide. Wheat is sensitive to waterlogging. Serrate^® (Syngenta) is a selective herbicide controlling annual grass and broadleaf weeds for use in wheat. To extend the existing information about the physiological effects of selective herbicides (Serrate^® in particular) and subsequent waterlogging in wheat, we monitored phenotype alterations and examined key enzymatic and non-enzymatic antioxidant defense systems together with typical oxidative stress biomarkers. Seventeen-day-old wheat (Triticum asetivum L., cv. Sadovo-1) plants were sprayed with Serrate^®; 72 h later, waterlogging was applied for 7 days, and then seedlings were left to recover for 96 h. The herbicide did not alter plant phenotype and increased antioxidant defense, along with H₂O₂ content, confirming the wheat's tolerance to Serrate^®. Evident yellowing and wilting of the leaves were observed at 96 h of recovery in waterlogged wheat, which were stronger in plants subjected to Serrate^® + waterlogging. Waterlogging alone and herbicide + waterlogging gradually enhanced the content of stress markers (malondialdehyde, proline, and H₂O₂), non-enzymatic antioxidants (low-molecular thiols and total phenolics), and the activity of superoxide dismutase, guaiacol peroxidase, and glutathione reductase. The effects of herbicide + waterlogging were stronger than those of waterlogging alone even during recovery, suggesting that Serrate^® interacted synergistically with the subsequently applied flooding.