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Neutral Re(I) Complex Platform for Live Intracellular Imaging.

Todd A Gillam1,2, Chiara Caporale3, Robert D Brooks1

  • 1UniSA Clinical and Health Sciences, University of South Australia, North Tce, Adelaide, South Australia 5000, Australia.

Inorganic Chemistry
|July 2, 2021
PubMed
Summary

Neutral rhenium(I) complexes offer a versatile platform for developing novel cell imaging agents. These neutral complexes, unlike cationic ones, show distinct intracellular localization within the endosomal/lysosomal network.

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Area of Science:

  • Inorganic Chemistry
  • Bioinorganic Chemistry
  • Materials Science

Background:

  • Luminescent metal complexes are crucial for developing effective cell imaging agents.
  • Cationic metal complexes often exhibit organelle-specific accumulation, limiting their application.
  • Neutral complexes provide a charge-independent platform for designing advanced imaging agents.

Purpose of the Study:

  • To synthesize neutral rhenium(I) complexes as a platform for bioconjugated imaging agents.
  • To create and characterize novel carbohydrate-conjugated rhenium(I) complexes.
  • To evaluate the utility of these neutral complexes for live cell imaging.

Main Methods:

  • Synthesis of a neutral Re(I) complex (ReAlkyne) functionalized for conjugation.
  • Cu(I)-catalyzed azide-alkyne cycloaddition (Click Chemistry) to attach four different carbohydrates.
  • Comprehensive physical and optical property evaluation.
  • Live cell imaging studies using H9c2 cardiomyoblasts.

Main Results:

  • Successful synthesis of a neutral ReAlkyne platform and four carbohydrate-conjugated derivatives.
  • Characterization of the photophysical properties of the synthesized complexes.
  • Demonstrated live cell imaging capabilities of the neutral complexes in H9c2 cells.
  • Observed intracellular localization within the endosomal/lysosomal network, distinct from mitochondrial localization of cationic complexes.

Conclusions:

  • Neutral Re(I) complexes are valuable platforms for bioconjugated cell imaging agents.
  • Carbohydrate conjugation via click chemistry yields versatile imaging agents.
  • The charge-neutrality of these complexes dictates their endosomal/lysosomal localization, offering a predictable distribution pattern.