Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Oct 30, 2025

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

High-resolution characterization of gene function using single-cell CRISPR tiling screen.

Lu Yang¹, Anthony K N Chan¹, Kazuya Miyashita¹

¹Department of Systems Biology, Beckman Research Institute, City of Hope, Duarte, CA, USA.

Nature Communications

|July 2, 2021

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

FATP2-mediated lipid metabolism enhances chimeric antigen receptor T-cell therapy resistance in B-cell acute lymphoblastic leukemia.

Leukemia·2026

Same author

Discovery of circadian clock activators with anti-obesity efficacy via suppression of adipocyte development and hypertrophy.

Molecular therapy : the journal of the American Society of Gene Therapy·2026

Same author

Inhibition of ADSS2-mediated de novo AMP biosynthesis re-sensitizes acute myeloid leukemia to BH3 mimetics.

Nature cancer·2026

Same author

AI-Driven Design of High Affinity Biomolecule-Drug Conjugates for Gynecological Cancer Therapy: An Up-to-Date Narrative Review.

Cancers·2026

Same author

Engineering nanozymes for cancer theranostics: Chemical design, catalytic intelligence, and translational oncology frontiers.

Translational oncology·2026

Same author

Targeting the METTL1/m7G axis as a therapeutic strategy in myeloid leukemia.

Blood·2026

Same journal

Sub1 contributes to heart failure with preserved ejection fraction driven by aging in mice.

Nature communications·2026

Same journal

The BRCA1-A complex restricts replication fork reversal-dependent DNA repair in ATM deficient cells.

Nature communications·2026

Same journal

Signaling downstream of tumor-stroma interaction regulates mucinous colorectal adenocarcinoma apicobasal polarity.

Nature communications·2026

Same journal

Click-polymerized polyenamine membranes for efficient lithium extraction.

Nature communications·2026

Same journal

Joint trajectories of brain atrophy, white matter hyperintensities and cognition quantify brain maintenance.

Nature communications·2026

Same journal

Proton shuttling at electrochemical interfaces under alkaline hydrogen evolution.

Nature communications·2026

See all related articles

This summary is machine-generated.

This study introduces sc-Tiling, a CRISPR gene editing method to map functional gene regions. It reveals how specific DNA sequences within genes control cancer gene activity and therapeutic outcomes.

Area of Science:

Genomics
Molecular Biology
Cancer Research

Background:

Understanding gene regulation is key for developing targeted cancer therapies.
CRISPR gene editing has advanced functional genomics but often focuses on single genes.
Single-cell RNA sequencing (sc-RNAseq) allows monitoring gene expression at high resolution.

Purpose of the Study:

To present sc-Tiling, a novel method integrating CRISPR tiling screens with single-cell transcriptomics.
To identify functional domains and regulatory mechanisms within gene-coding regions.
To understand how these mechanisms influence gene activity and response to cancer therapy.

Main Methods:

CRISPR gene tiling of coding exons to create high-density mutations.
Integration with single-cell RNA sequencing for transcriptomic profiling.

More Related Videos

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Published on: June 6, 2020

Related Experiment Videos

Last Updated: Oct 30, 2025

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Published on: June 6, 2020

Analysis of protein structures to correlate with functional observations.

Main Results:

sc-Tiling successfully maps functional regions within genes.
Identified regulatory mechanisms within coding regions impacting gene activity.
Demonstrated potential for predicting therapeutic response based on genetic perturbations.

Conclusions:

sc-Tiling offers a powerful approach to dissect gene function at a granular level.
This method advances the understanding of gene regulation in cancer.
Provides a foundation for novel therapeutic strategies targeting specific gene elements.