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Bioactive Compounds of Raspberry Oil Emulsions Induced Oxidative Stress via Stimulating the Accumulation of Reactive

Magdalena Grajzer1, Benita Wiatrak2,3, Tomasz Gębarowski3

  • 1Department of Food Science and Dietetics, Wroclaw Medical University, Borowska 211, Wroclaw 50-556, Poland.

Oxidative Medicine and Cellular Longevity
|July 2, 2021
PubMed

Insights

Raspberry seed oils show potential as non-toxic cancer therapy support. Supercritical CO2-extracted raspberry oil (ROSCO2) effectively targeted cancer cells, inducing DNA damage and free radical production.

Area of Science:

  • Natural product chemistry
  • Cancer biology
  • Pharmacology

Background:

  • Growing interest in natural compounds for cancer therapy.
  • Seed oils, like raspberry oil, are complex natural mixtures.
  • Raspberry oils offer potential as supportive cancer treatments.

Purpose of the Study:

  • Evaluate chemical characteristics of two raspberry oils: cold-pressed (ROCOP) and supercritical CO2-extracted (ROSCO2).
  • Assess the cancer cell suppression potential of raspberry oil emulsions.
  • Determine cytotoxicity against various human cancer cell lines and normal fibroblasts.

Main Methods:

  • Preparation of raspberry oil emulsions at concentrations of 0.5-10%.
  • Cytotoxicity assays on colon adenocarcinoma (LoVo, LoVo/DX), breast cancer (MCF7, MCF7/DX), and lung cancer (A549) cell lines.
  • Assessment of normal human dermal fibroblasts (NHDFs) for toxicity.
  • Measurement of free radical production and DNA strand damage.

Main Results:

  • Increasing concentrations of raspberry oil emulsions inhibited cancer cell viability and proliferation across all tested lines.
  • Supercritical CO2-extracted raspberry oil (ROSCO2) demonstrated potent effects, particularly against doxorubicin-resistant cell lines.
  • ROSCO2 induced significant free radical production and DNA strand damage in LoVo and MCF7 cells.
  • Both oils showed varying degrees of potency depending on the cancer type and oil tested.

Conclusions:

  • Raspberry oil emulsions exhibit dose-dependent inhibition of cancer cell growth.
  • ROSCO2 effectively targets cancer cell vulnerabilities, suggesting a promising therapeutic mechanism.
  • Both ROCOP and ROSCO2 can serve as non-toxic supportive agents in the therapy of specific human cancers.

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