[Advances in capillary-based immobilized enzyme microreactor based on DNA-directed immobilization]

Area of Science:

• Biochemistry and analytical chemistry
• Enzyme kinetics and analysis
• Nanotechnology and biomaterials

Background:

• Enzymatic activity is crucial for life processes; altered activity indicates disease, necessitating advanced analysis methods.
• Capillary electrophoresis (CE) offers high efficiency, speed, and minimal sample requirements for enzyme analysis.
• On-line CE modes, particularly immobilized enzyme microreactors (CE-IMERs), enhance enzyme stability, reusability, and automation.

Purpose of the Study:

• To explore DNA-directed immobilization (DDI) as a method for preparing high-performance CE-IMERs.
• To improve enzyme stability, reusability, and automation in capillary electrophoresis-based enzyme analysis.
• To present preliminary research on DDI for novel IMER development.

Main Methods:

• Enzyme immobilization within capillaries using DNA-directed immobilization (DDI).
• Utilizing complementary DNA base pairing for specific and mild biomacromolecule attachment.
• Developing enzyme microarrays with enhanced mass transfer and enzyme-substrate contact.

Main Results:

• DDI enables enzyme immobilization under mild physiological conditions, preserving enzyme activity and conformation.
• DDI offers reversible immobilization, carrier regeneration, and reduced preparation costs.
• DDI-based CE-IMERs show potential for improved stability, reusability, and automation.

Conclusions:

• DNA-directed immobilization is an ideal method for preparing immobilized enzyme microreactors for capillary electrophoresis.
• Further research on DDI-based IMERs can lead to more efficient catalytic systems and wider applications in enzyme analysis.
• Developing novel IMERs using DNA nanotechnology and nanomaterials is crucial for advancing CE-IMER technology.