Related Experiment Video
Updated: Oct 30, 2025
The MPLEx Protocol for Multi-omic Analyses of Soil Samples
Published on: May 30, 2018
[A liquid chromatography-mass spectrometry-based metabolomic study on the changes of intracellular lipid metabolism
Yibao Jin1, Shangfu Li2, Jue Wang1
1Shenzhen Institute for Drug Control, Shenzhen Key Laboratory of Drug Quality Standard Research, Shenzhen 518057, China.
Abstract:
Pokemon, a transcriptional repressor, inhibits the transcription of tumor suppressor genes and promotes tumor formation by affecting chromatin recombination or binding directly to the tumor suppressor genes. In this study, the mechanism behind the Pokemon regulation of liver cancer cell metabolism was evaluated using liquid chromatography-mass spectrometry (LC-MS)-based metabolomics. Pokemon-overexpressing HL7702 cells were obtained by lipofection. The cells were collected at different time points after transfection and their intracellular metabolites were analyzed using an LC-MS-based metabolomic method. Based on the results of a multivariate statistical analysis, candidate metabolites with significant differences were selected, and 36 of them were confirmed by database and MS/MS analysis. These metabolites were primarily involved in lipid synthesis by KEGG database searches. Further analysis indicated that both the acetyl-coenzyme carboxylase and the fatty acid synthase were activated in the lipid synthesis pathway. The results show that Pokemon can affect cell metabolism by activating the lipid synthesis pathways in cells.
Insights
Pokemon protein promotes liver cancer by activating lipid synthesis pathways. This study used metabolomics to reveal how Pokemon influences cancer cell metabolism, identifying key metabolic targets for potential therapies.
Area of Science:
- Molecular Biology
- Cancer Research
- Metabolomics
Background:
- Pokemon is a transcriptional repressor implicated in tumor formation by inhibiting tumor suppressor genes.
- The role of Pokemon in regulating cancer cell metabolism, particularly in liver cancer, remains largely unexplored.
Purpose of the Study:
- To investigate the mechanism by which Pokemon regulates liver cancer cell metabolism.
- To identify specific metabolic pathways affected by Pokemon overexpression in liver cells.
Main Methods:
- Utilized liquid chromatography-mass spectrometry (LC-MS)-based metabolomics to analyze intracellular metabolites.
- Established Pokemon-overexpressing HL7702 cells via lipofection for experimental analysis.
- Employed multivariate statistical analysis and MS/MS for metabolite identification and confirmation.
Main Results:
- Identified 36 significantly different metabolites, primarily involved in lipid synthesis pathways.
- Confirmed activation of key enzymes in lipid synthesis, including acetyl-CoA carboxylase and fatty acid synthase.
- Demonstrated that Pokemon overexpression alters cellular metabolism by promoting lipid synthesis.
Conclusions:
- Pokemon plays a significant role in regulating liver cancer cell metabolism.
- Pokemon activates lipid synthesis pathways, contributing to metabolic alterations in cancer cells.
- Targeting Pokemon-mediated lipid synthesis may offer a novel therapeutic strategy for liver cancer.
More Related Videos
07:55Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
Published on: June 21, 2019
14:42Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
Published on: September 23, 2021