Related Experiment Video
Updated: Oct 30, 2025

Synthesis and Purification of Iodoaziridines Involving Quantitative Selection of the Optimal Stationary Phase for Chromatography
Published on: May 16, 2014
[Research progress on core-shell silica stationary phases for high performance liquid chromatography]
Hongjun Xia1,2, Jiawei Liu1, Quan Bai1
1Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Institute of Modern Separation Science, Key Lab of Modern Separation Science in Shaanxi Province, Northwest University, Xi'an 710069, China.
Abstract:
Fast and efficient separation by using high performance liquid chromatography (HPLC) is challenging for a wide range of samples. In recent years, core-shell silica microspheres have been widely investigated and used for highly efficient and fast separation under reasonably low pressure for small solutes, biopolymers, and complex samples. In this paper, the mechanism for fast separation and preparation methods of core-shell silica microspheres are introduced, along with their application for HPLC-based separation of small molecules, peptides, and biological macromolecules in HPLC. The developing trends of using core-shell microspheres in HPLC are also presented.
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Silica Gel Column Chromatography: Overview
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
Gas Chromatography: Types of Columns and Stationary Phases
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
High-Performance Liquid Chromatography: Elution Process
High-Performance Liquid Chromatography: Instrumentation
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...

