[A polymer resin surface polymerization-based hydroxide-selective anion exchange stationary phase]

A polystyrene-divinylbenzene (PS-DVB) microspheres-based hydroxide-selective anion exchange stationary phase is described. This stationary phase is obtained by the surface polymerization of an allyl glycidyl ether (AGE) and the pendant vinyl groups onto the surface of PS-DVB, followed by open-ring of the epoxy groups using an alkylol amine (abbreviated as P@A-M). Both alkylol amines were compared in terms of separation performance of the stationary phase. Scanning electron microscopy, infrared spectroscopy, and elemental analyses were carried out to characterize the stationary phase. The results indicated that the quaternary amine group was successfully introduced onto the surface of the PS-DVB microspheres, and that there was no obvious change in the physicochemical properties of the resin during the surface polymerization reaction. The P@A-M phase showed high hydroxide-selectivity and good separation performance (resolution>1.5) as well as high running stability (relative standard deviation of retention times<1.13%). The utility of the P@A-M phase was demonstrated by using it for the determination of inorganic anions in the tea.