Related Experiment Video
Updated: Oct 30, 2025

Functionalized Spirocyclic Heterocycle Synthesis and Cytotoxicity Assay
Published on: February 9, 2021
Discovery of heterocyclic substituted dihydropyrazoles as potent anticancer agents
Chuan-Huizi Chen1, Yuan Jiang1, Runfang Wu2
1School of Chinese Materia Medica, Yunnan University of Chinese Medicine, Kunming 650500, PR China.
Abstract:
In this work, a series of novel heterocyclic substituted dihydropyrazole derivatives have been prepared, and in vitro anticancer activity against a panel of human tumor cell lines by SRB were evaluated. The results indicated that piperazine substituted dihydropyrazole derivatives exhibited superior anticancer activity than that of other compounds. Especially, compounds 4g, 4h, 4l, 4m, 4o, 6g, 6j and 6l showed potent antitumor activity. Further mechanism study demonstrated that compound 4o could induce G2/M arrest in HCC1806 cell and p21 accumulation significantly.
Related Concept Videos
Five-Membered Heterocyclic Aromatic Compounds: Overview
Aryldiazonium Salts to Azo Dyes: Diazo Coupling
Basicity of Heterocyclic Aromatic Amines
Chemotherapy-Induced Nausea and Vomiting: Dopamine Receptor Antagonists
Phenothiazines, such as prochlorperazine...
Inhibition of Cdk Activity
Diazonium Group Substitution: –OH and –H

