Electrochemiluminescence aptasensor for lincomycin antigen detection by using a SnO₂/chitosan/g-C₃N₄ nanocomposite

In this paper, hydrothermal method was used for the synthesis of SnO₂ quantum dots (QDs). The prepared SnO₂ QDs have a uniform particle size distribution and good electrochemiluminescence (ECL) property. Then the prepared SnO₂ QDs was combined with graphene-like carbon nitride (g-C₃N₄) through chitosan to form SnO₂/chitosan/g-C₃N₄ nanocomposite and used for detecting the lincomycin. The characteristics of SnO₂/chitosan/g-C₃N₄ nanocomposite were presented by transmission electron microscopy (TEM), X-ray diffraction (XRD) and energy dispersive spectroscopy (EDS), and the analytical results proving that the nanocomposite was prepared successfully. In this strategy, the SnO₂/chitosan/g-C₃N₄ nanocomposite was acted as the substrate of aptasensor. Then, SH-DNA (aptamer DNA) was assembled on the surface of electrode, after 6-mercaptohexanol (MCH) blocked the unbound sites of the electrode surface, ferrocene-DNA (Fc-DNA) was incubated on the electrode surface through base complementation with aptamer DNA. In the absence of lincomycin, due to the low conductivity of Fc-DNA and the photo-excited energy electron transfer, the ECL signal was quenched. In the presence of lincomycin, the aptamer DNA was specific binding with lincomycin, and ferrocene-DNA (Fc-DNA) was detached from the surface of aptasensor electrode, generating an obviously enhancement of ECL signal. To ensure the accuracy of the data, each electrode runs continuously for 3600 s. Under optimal experimental conditions, the detection range of the aptasensor was 0.10 ng mL^-1 - 0.10 mg mL^-1, and the detection limit was 0.028 ng mL^-1. In addition, the aptasensor has good stability and reproducibility, and also provided a hopeful device for all kinds of other protein target.