Related Experiment Video For Carbonic-anhydrase IX
Updated: Oct 30, 2025

An Orthotopic Mouse Model of Anaplastic Thyroid Carcinoma
Published on: April 17, 2013
Carbonic-anhydrase IX expression is increased in thyroid cancer tissue and represents a potential therapeutic target
Jennifer Schmidt1, Elsie Oppermann1, Roman A Blaheta2
1Hospital of the Goethe University Frankfurt, Department of General, Visceral and Transplant Surgery, Theodor-Stern-Kai 7, 60590, Frankfurt am Main, Germany.
Abstract:
The expression of Carbonic-anhydrase IX (CAIX) in thyroid cancer (TC) subtypes was determined and its role in cancer cell growth and tumor-initiating cells (TICs) investigated. Immunohistochemistry in 114 TC patients revealed that CAIX expression was increased in tumor specimens of papillary, follicular and anaplastic TCs compared to normal thyroid tissue. Clinicopathological data indicated that lymph node metastases were more frequent in patients with high CAIX expression. The Cancer Genome Atlas database analysis demonstrated that a strong CAIX-mRNA expression was associated with advanced tumor stages and poor survival in TCs. In TC cell lines, CAIX expression was elevated in tumorspheres compared to monolayer cultures and was associated with an increased expression of stemness markers. Genetic knockdown or pharmacological inhibition of CAIX suppressed cell proliferation and the TIC ability to form tumorspheres by an induction of apoptosis and cell-cycle arrest. These findings suggest CAIX as a potential prognostic marker and a therapeutic target for thyroid cancer.
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