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CUBIC-plus: An optimized method for rapid tissue clearing and decolorization.

Zhengyu Ren1, Yuzheng Wu2, Zhenning Wang3

  • 1School of Pharmaceutical Science, University of South China, Hengyang, 421001, China; Guangzhou Institute of Respiratory Disease & China State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510182, China.

Biochemical and Biophysical Research Communications
|July 3, 2021
PubMed
Summary
This summary is machine-generated.

The Advanced CUBIC clearing method was optimized to improve efficiency for high-resolution 3D imaging. The new CUBIC-Plus method significantly reduces clearing time for diverse organs, enhancing data acquisition.

Keywords:
3D imagingCUBICDecolorizationOptimizationTissue clearingUltrasound

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Area of Science:

  • Biological Imaging
  • Tissue Clearing Techniques
  • Microscopy

Background:

  • Advanced CUBIC is a low-toxicity method for clearing organs and whole bodies for 3D imaging.
  • However, its long experimental cycle limits data acquisition efficiency.
  • Optimizing clearing time is crucial for high-resolution biological imaging.

Purpose of the Study:

  • To optimize the Advanced CUBIC clearing method for improved efficiency.
  • To reduce the experimental cycle time for clearing diverse organs.
  • To enhance data acquisition for high-resolution 3D imaging.

Main Methods:

  • Tracked clarity changes in different organs using Advanced CUBIC to determine optimal clearing times.
  • Introduced ultrasound processing to accelerate the clearing process.
  • Developed a decolorization cocktail to further optimize clearing efficiency.

Main Results:

  • Identified shortest optimal transparency times for individual mouse organs.
  • Developed the optimized CUBIC-Plus method, significantly reducing clearing duration.
  • Achieved high-resolution 3D imaging of mouse organs with enhanced efficiency.

Conclusions:

  • CUBIC-Plus offers an efficient and optimized procedure for clearing diverse organs.
  • This method enables high-resolution 3D imaging with reduced experimental time.
  • The optimized protocol enhances overall data acquisition efficiency in biological imaging.