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Comparing High-Frequency With Monophasic Electroporation Protocols in an In Vivo Beating Heart Model
Eyal Heller1, Tomas Garcia-Sanchez2, Yonatan Moshkovits1
1Neufeld and Tamman Cardiovascular Research Institutes, Sheba Medical Center, Sackler School of Medicine, Tel Aviv University, Israel; Heart Center, Sheba Medical Center, Tel Hashomer, Israel.
Abstract:
This study compared monophasic 100-μs pulses with high-frequency electroporation (HF-EP) bursts using an in vivo animal model. Myocardial damage was evaluated by histologic analysis. Compared with 10 monophasic pulses, 20 bursts of HF-EP at 100 and 150 kHz were associated with less damage. However, when the number of HF-EP bursts was increased to 60, myocardial damage was comparable to that of the monophasic group. HF-EP protocols were associated with attenuated collateral muscle contractions. This study shows that HF-EP is feasible and effective and that pulse frequency has a significant effect on extent of ablation.
