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Recent advances in lineage tracing for the kidney
Yoshiharu Muto1, Benjamin D Humphreys2
1Division of Nephrology, Department of Medicine, Washington University in St. Louis School of Medicine, St. Louis, Missouri, USA.
Kidney International
|July 4, 2021
Summary
Lineage tracing techniques, crucial for understanding cell development and repair, have advanced significantly. Modern methods, including CRISPR-Cas9 and single-cell RNA-sequencing, enable comprehensive organism-wide lineage reconstruction, with potential kidney research applications.
Area of Science:
- Developmental Biology
- Genetics
- Regenerative Medicine
Background:
- Lineage tracing identifies cell progeny, initially for morphogenesis studies.
- Applications expanded to organ homeostasis and injury recovery.
- Modern techniques use reporter gene expression via DNA recombination.
Purpose of the Study:
- To review recent advancements in lineage tracing technologies.
- To outline potential applications of these technologies in kidney research.
Main Methods:
- Review of scientific literature on lineage tracing.
- Discussion of techniques like intersectional genetics and optical clearing.
- Focus on sequencing-based genomic lineage tracing using CRISPR-Cas9 and single-cell RNA-sequencing.
Main Results:
- Significant scientific advances in the last decade have impacted lineage tracing.
- CRISPR-Cas9 genetic scarring combined with single-cell RNA-sequencing offers comprehensive lineage tree reconstruction.
- New technologies enable detailed tracking of cell fates.
Conclusions:
- Recent lineage tracing technologies offer unprecedented resolution for biological research.
- These advanced methods hold significant promise for kidney research and understanding organ function and repair.
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