Related Experiment Video

Updated: Oct 30, 2025

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

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Splice-switching as cancer therapy

Amina Jbara1, Zahava Siegfried1, Rotem Karni1

  • 1Department of Biochemistry and Molecular Biology, Institute for Medical Research Israel-Canada, Hebrew University-Hadassah Medical School, Jerusalem, 91120, Israel.

Current Opinion in Pharmacology
|July 4, 2021
PubMed

Insights

RNA splicing modulation offers promising cancer therapies by targeting aberrant splicing. This study compares splice-switching oligonucleotides and CRISPR-Cas technology for cancer treatment, discussing challenges and progress.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Therapeutics

Background:

  • Alternative RNA splicing is frequently dysregulated in cancer, presenting a therapeutic target.
  • Recent advances in RNA splicing modulation for genetic diseases fuel optimism for cancer treatment applications.

Purpose of the Study:

  • To compare two novel therapeutic strategies for manipulating RNA splicing in cancer.
  • To evaluate the potential of splice-switching oligonucleotides and CRISPR-Cas technology in cancer therapy.

Main Methods:

  • Investigating splice-switching oligonucleotides for RNA splicing modulation.
  • Exploring CRISPR-Cas technology for targeting aberrant RNA splicing in cancer.

Main Results:

  • Both splice-switching oligonucleotides and CRISPR-Cas technology show promise for therapeutic manipulation of RNA splicing.
  • Challenges and limitations of these technologies in cancer therapy are being addressed.

Conclusions:

  • RNA splicing modulation represents a viable therapeutic strategy for cancer treatment.
  • Further research and development are needed to overcome challenges and implement these approaches effectively in clinical settings.

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