Fe(III)-mediated reversible catalytic activity of MoS₂ nanozymes for bisphosphonate drug sensing

Peroxidase-like activity of MoS₂ quantum dots (QDs) can be reversibly regulated by means of Fe³⁺/alendronate sodium (ALDS)-induced aggregation/disaggregation of the QDs in solution. Specifically, Fe³⁺ can selectively aggregate the MoS₂ QDs and thus greatly enhance their peroxidase-like activity, while such enhancement can be inhibited in the presence of ALDS owing to the competitive coordination of ALDS with Fe³⁺. By regulating the enzyme-like activity of MoS₂ QDs, different colorimetric signal of a typical substrate of horseradish peroxidase, 3,3΄,5,5΄-tetramethylbenzidine, can be measured in the presence of H₂O₂. Based on this mechanism, we develop a colorimetric approach for the determination of ALDS and further applied in quality control of pharmaceutical products, utilizing either smartphone or UV-vis spectrometer as a readout. This detection method is rapid and selective, where derivatization of ALDS before detection is not needed. Such a smartphone-based colorimetric detection platform is promising to be applied in point-of-care testing at home, small clinics, or underdeveloped regions.