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Updated: Oct 30, 2025

Generation of a RIP1 Knockout U937 Cell Line Using the CRISPR-Cas9 System
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The role of RIPK3 in liver mitochondria bioenergetics and function

Tawhidul Islam1, Marta B Afonso1, Cecília M P Rodrigues1

  • 1Faculty of Pharmacy, Research Institute for Medicines (iMed.ULisboa), Universidade de Lisboa, Lisbon, Portugal.

European Journal of Clinical Investigation
|July 5, 2021
PubMed
Abstract

Insights

Receptor-interacting protein kinase 3 (RIPK3) regulates necroptosis and impacts mitochondrial function. Understanding RIPK3

Area of Science:

  • Cellular Biology
  • Molecular Biology
  • Hepatology

Background:

  • Receptor-interacting protein kinase 3 (RIPK3) is central to necroptosis, an inflammatory cell death pathway.
  • Necroptosis plays a role in chronic liver diseases like nonalcoholic fatty liver disease and nonalcoholic steatohepatitis.

Purpose of the Study:

  • To review the literature on the role of RIPK3 in mitochondrial bioenergetics.
  • To explore the potential translational applications of targeting RIPK3 in liver disease.

Main Methods:

  • This study is a narrative review based on a PubMed literature search.

Main Results:

  • RIPK3 activation involves post-transcriptional modifications and forms the necrosome complex, potentially including mitochondrial components.
  • RIPK3 influences mitochondrial function by regulating reactive oxygen species, metabolic enzymes, respiratory chain activity, biogenesis, and fatty acid oxidation.

Conclusions:

  • Mitochondrial dysfunction and RIPK3-mediated necroptosis are implicated in chronic liver disease pathogenesis.
  • Further understanding of RIPK3's role in mitochondrial bioenergetics is crucial for potential therapeutic strategies.
Keywords:
NAFLDNASHRIPK3mitochondrianecroptosis

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